Application
A.Western blot analysis on (A) HeLa, (B) Jurkat, (C) Raji and (D) 293T cell lysates using anti-RBBP4 RabMAb (Cat. #AJ1684b), dilution 1:20,000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-RBBP4 RabMAb (Cat. #AJ1684b).| Product Name | RBBP4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBBP4, RBAP48, Histone-binding protein RBBP4, Chromatin assembly factor I p48 subunit;CAF-I 48 kDa subunit;CAF-I p48;Nucleosome-remodeling factor subunit RBAP48 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3412 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:20000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RBAP48 |
|---|---|
| Alternative Names | RBBP4 RBAP48 Histone-binding protein RBBP4 Chromatin assembly factor I p48 subunit CAF-I 48 kDa subunit CAF-I p48 Nucleosome-remodeling factor subunit RBAP48 |
| Molecular Weight(MW) | 47656 Da |
| Function | Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex |
| Tissue Specificity | A synthetic peptide corresponding to residues in human RBBP4 was used as an immunogen. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5928 |
|---|
Application
A.Western blot analysis on (A) HeLa, (B) Jurkat, (C) Raji and (D) 293T cell lysates using anti-RBBP4 RabMAb (Cat. #AJ1684b), dilution 1:20,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-RBBP4 RabMAb (Cat. #AJ1684b).| Application Notes | WB:1:20000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
