Application
Western blot analysis of hRBBP4 N-term I63 in 293 cell line lysates (35ug/lane). RBBP4 (arrow) was detected using the purified Pab.Application
Western blot analysis of RBBP4 (arrow) using rabbit polyclonal RBBP4 Antibody (N-term I63) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RBBP4 gene.| Product Name | RBBP4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBBP4; RBAP48; Histone-binding protein RBBP4; Chromatin assembly factor 1 subunit C; Chromatin assembly factor I p48 subunit; Nucleosome-remodeling factor subunit RBAP48; Retinoblastoma-binding protein 4; Retinoblastoma-binding protein p48 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RBAP48 |
|---|---|
| Alternative Names | RBBP4 RBAP48 Histone-binding protein RBBP4 Chromatin assembly factor 1 subunit C Chromatin assembly factor I p48 subunit Nucleosome-remodeling factor subunit RBAP48 Retinoblastoma-binding protein 4 Retinoblastoma-binding protein p48 |
| Molecular Weight(MW) | 47656 Da |
| Function | Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex |
| Tissue Specificity | This RBBP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human RBBP4. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5928 |
|---|
Application
Western blot analysis of hRBBP4 N-term I63 in 293 cell line lysates (35ug/lane). RBBP4 (arrow) was detected using the purified Pab.
Application
Western blot analysis of RBBP4 (arrow) using rabbit polyclonal RBBP4 Antibody (N-term I63) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RBBP4 gene.| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
