Application
RBBP8 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RBBP8 antibody detected the RBBP8 protein (arrow).| Product Name | RBBP8 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBBP8; CTIP; DNA endonuclease RBBP8; CtBP-interacting protein; Retinoblastoma-binding protein 8; Retinoblastoma-interacting protein and myosin-like; Sporulation in the absence of SPO11 protein 2 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CTIP |
|---|---|
| Alternative Names | RBBP8 CTIP DNA endonuclease RBBP8 CtBP-interacting protein Retinoblastoma-binding protein 8 Retinoblastoma-interacting protein and myosin-like Sporulation in the absence of SPO11 protein 2 homolog |
| Molecular Weight(MW) | 101942 Da |
| Function | Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage Promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations |
| Tissue Specificity | This RBBP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 762-792 amino acids from the C-terminal region of human RBBP8. |
| Cellular Localization | Nucleus. Note=Associates with sites of DNA damage in S/G2 phase. Ubiquitinated RBBP8 binds to chromatin following DNA damage |
| Entrez Gene | 5932 |
|---|---|
| Nucleotide Accession | NP_976037.1 NP_976036.1 NP_002885.1 |
Application
RBBP8 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RBBP8 antibody detected the RBBP8 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
