Application
RBM14 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RBM14 antibody detected the RBM14 protein (arrow).Application
RBM14 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBM14 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
RBM14 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RBM14 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBM14; SIP; RNA-binding protein 14; Paraspeckle protein 2; RNA-binding motif protein 14; RRM-containing coactivator activator/modulator; Synaptotagmin-interacting protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SIP |
|---|---|
| Alternative Names | RBM14 SIP RNA-binding protein 14 Paraspeckle protein 2 RNA-binding motif protein 14 RRM-containing coactivator activator/modulator Synaptotagmin-interacting protein |
| Molecular Weight(MW) | 69492 Da |
| Function | Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1 |
| Tissue Specificity | Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Note=In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles |
| Entrez Gene | 10432 |
|---|---|
| Nucleotide Accession | NP_006319.1 |
Application
RBM14 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RBM14 antibody detected the RBM14 protein (arrow).
Application
RBM14 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBM14 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
RBM14 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
