Application
RCAN1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RCAN1 antibody detected the RCAN1 protein (arrow).Application
Western blot analysis of RCAN1 (arrow) using rabbit polyclonal RCAN1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCAN1 gene.Application
Fluorescent confocal image of A431 cell stained with RCAN1 Antibody (N-term)(Cat#AP16791a).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RCAN1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RCAN1 immunoreactivity is localized to Cytoplasm significantly.| Product Name | RCAN1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RCAN1; ADAPT78; CSP1; DSC1; DSCR1; Calcipressin-1; Adapt78; Down syndrome critical region protein 1; Myocyte-enriched calcineurin-interacting protein 1; Regulator of calcineurin 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ADAPT78 CSP1 DSC1 DSCR1 |
|---|---|
| Alternative Names | RCAN1 ADAPT78 CSP1 DSC1 DSCR1 Calcipressin-1 Adapt78 Down syndrome critical region protein 1 Myocyte-enriched calcineurin-interacting protein 1 Regulator of calcineurin 1 |
| Molecular Weight(MW) | 28079 Da |
| Function | Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development |
| Tissue Specificity | Highly expressed heart, brain and skeletal muscle. Also expressed in all other tissues |
| Entrez Gene | 1827 |
|---|---|
| Nucleotide Accession | NP_004405.3 NP_981963.1 |
Application
RCAN1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RCAN1 antibody detected the RCAN1 protein (arrow).
Application
Western blot analysis of RCAN1 (arrow) using rabbit polyclonal RCAN1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCAN1 gene.
Application
Fluorescent confocal image of A431 cell stained with RCAN1 Antibody (N-term)(Cat#AP16791a).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RCAN1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RCAN1 immunoreactivity is localized to Cytoplasm significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
