Application
Total lysates (50ug per lane) from Hela cells untransfected (U) or transfected with negative siRNA control or Rent1 siRNAs of 10nm, 50nm to 100nm. Immunostaining was with polyclonal rabbit Rent1 antibody(AP1905c, 1:1000) overnight. The membrane was incubated with donkey anti-rabbit antibody(1:2000) for 1 hour. Antibody-reactive bands were revealed by chemiluminescence. Rent1 protein migrated between 100KD and 150KD. Data courtesy of Dr. Jingqiong Kang, Department of Neurology, Vanderbilt University.| Product Name | RENT1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UPF1; KIAA0221; RENT1; Regulator of nonsense transcripts 1; ATP-dependent helicase RENT1; Nonsense mRNA reducing factor 1; Up-frameshift suppressor 1 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0221 RENT1 |
|---|---|
| Alternative Names | UPF1 KIAA0221 RENT1 Regulator of nonsense transcripts 1 ATP-dependent helicase RENT1 Nonsense mRNA reducing factor 1 Up-frameshift suppressor 1 homolog |
| Molecular Weight(MW) | 124345 Da |
| Function | RNA-dependent helicase and ATPase required for nonsense- mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1- eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm. Cytoplasm, P-body. Nucleus. Note=Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm |
| Entrez Gene | 5976 |
|---|
Application
Total lysates (50ug per lane) from Hela cells untransfected (U) or transfected with negative siRNA control or Rent1 siRNAs of 10nm, 50nm to 100nm. Immunostaining was with polyclonal rabbit Rent1 antibody(AP1905c, 1:1000) overnight. The membrane was incubated with donkey anti-rabbit antibody(1:2000) for 1 hour. Antibody-reactive bands were revealed by chemiluminescence. Rent1 protein migrated between 100KD and 150KD. Data courtesy of Dr. Jingqiong Kang, Department of Neurology, Vanderbilt University.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
