Application
RET (Cat. #AM1869a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RET antibody detected the RET protein (arrow).Application
RET Monoclonal( Ascites) (Cat. #AM1869a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RET Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of RET Antibody (Ascites)(Cat#AM1869a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Product Name | RET Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RET; CDHF12; CDHR16; PTC; RET51; Proto-oncogene tyrosine-protein kinase receptor Ret; Cadherin family member 12; Proto-oncogene c-Ret; Soluble RET kinase fragment; Extracellular cell-membrane anchored RET cadherin 120 kDa fragment |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 188CT11.2.3 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:500~16000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDHF12 CDHR16 PTC RET51 |
|---|---|
| Alternative Names | RET CDHF12 CDHR16 PTC RET51 Proto-oncogene tyrosine-protein kinase receptor Ret Cadherin family member 12 Proto-oncogene c-Ret Soluble RET kinase fragment Extracellular cell-membrane anchored RET cadherin 120 kDa fragment |
| Molecular Weight(MW) | 124319 Da |
| Function | Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut- associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration |
| Tissue Specificity | This RET Monoclonal antibody was raised using purified His-tagged recombinant human RET. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein |
| Entrez Gene | 5979 |
|---|---|
| Nucleotide Accession | NP_065681.1 NP_066124.1 |
Application
RET (Cat. #AM1869a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RET antibody detected the RET protein (arrow).
Application
RET Monoclonal( Ascites) (Cat. #AM1869a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RET Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of RET Antibody (Ascites)(Cat#AM1869a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:500~16000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
