Application
A. Western blot analysis on (A) Raji and (B) HeLa cell lysates using anti-REST RabMAb (Cat. #AJ1686a), dilution 1:2000.| Product Name | Rest Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2436Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NRSF, XBR |
|---|---|
| Alternative Names | REST NRSF XBR RE1-silencing transcription factor Neural-restrictive silencer factor X2 box repressor |
| Molecular Weight(MW) | 121872 Da |
| Function | Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier |
| Tissue Specificity | Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 5978 |
|---|
Application
A. Western blot analysis on (A) Raji and (B) HeLa cell lysates using anti-REST RabMAb (Cat. #AJ1686a), dilution 1:2000.| Application Notes | WB:1:1000~2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
