Application
REST Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the REST antibody detected the REST protein (arrow).| Product Name | REST Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NRSF, XBR |
|---|---|
| Alternative Names | REST NRSF XBR RE1-silencing transcription factor Neural-restrictive silencer factor X2 box repressor |
| Molecular Weight(MW) | 121872 Da |
| Function | Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier |
| Tissue Specificity | Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 5978 |
|---|---|
| Nucleotide Accession | NP_005603.3 NP_001180437.1 |
Application
REST Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the REST antibody detected the REST protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
