Application
A. Western blot analysis on (A) Raji and (B) SH-SY5Y cell lysates using anti-Rheb RabMAb (Cat. #AJ1687a), dilution 1:2000.Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Rheb RabMAb (Cat. #AJ1687a).| Product Name | Rheb Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2971 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RHEB2 |
|---|---|
| Alternative Names | RHEB RHEB2 GTP-binding protein Rheb Ras homolog enriched in brain |
| Molecular Weight(MW) | 20497 Da |
| Function | Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity |
| Tissue Specificity | Ubiquitous. Highest levels observed in skeletal and cardiac muscle |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side (Potential) |
| Entrez Gene | 6009 |
|---|
Application
A. Western blot analysis on (A) Raji and (B) SH-SY5Y cell lysates using anti-Rheb RabMAb (Cat. #AJ1687a), dilution 1:2000.
Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Rheb RabMAb (Cat. #AJ1687a).| Application Notes | WB:1:5000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
