Application
Western blot analysis of anti-RHEB Antibody (Center) in CEM cell line lysates (35ug/lane). RHEB(arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RHEB antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | RHEB Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RHEB2 |
|---|---|
| Alternative Names | RHEB RHEB2 GTP-binding protein Rheb Ras homolog enriched in brain |
| Molecular Weight(MW) | 20497 Da |
| Function | Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity |
| Tissue Specificity | Ubiquitous. Highest levels observed in skeletal and cardiac muscle |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side (Potential) |
| Entrez Gene | 6009 |
|---|
Application
Western blot analysis of anti-RHEB Antibody (Center) in CEM cell line lysates (35ug/lane). RHEB(arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RHEB antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
