Application
RIPK1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RIPK1 antibody detected the RIPK1 protein (arrow).Application
RIPK1 Antibody (N-term) (AP13893a)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RIPK1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | RIPK1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RIP, RIP1 |
|---|---|
| Alternative Names | RIPK1 RIP RIP1 Receptor-interacting serine/threonine-protein kinase 1 Cell death protein RIP Receptor-interacting protein 1 Serine/threonine-protein kinase RIP |
| Molecular Weight(MW) | 75931 Da |
| Function | Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex |
| Tissue Specificity | This RIPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the N-terminal region of human RIPK1. |
| Cellular Localization | Cytoplasm. Cell membrane (By similarity). |
| Entrez Gene | 8737 |
|---|---|
| Nucleotide Accession | NP_003795.2 |
Application
RIPK1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RIPK1 antibody detected the RIPK1 protein (arrow).
Application
RIPK1 Antibody (N-term) (AP13893a)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RIPK1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
