Application
Western Blot analysis of RNASEH2A expression in transfected 293T cell line by RNASEH2A monoclonal antibody (M01), clone 3G5-F5.Lane 1: RNASEH2A transfected lysate(33.4 KDa).Lane 2: Non-transfected lysate.Application
Western blot analysis of RNASEH2A over-expressed 293 cell line, cotransfected with RNASEH2A Validated Chimera RNAi ( Cat # H00010535-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNASEH2A monoclonal antibody (M01), clone 3G5-F5 (Cat # AT3653a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Product Name | RNASEH2A Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JUNB, RNASEHI, RNHIA, RNHLribonuclease H2, large subunit |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3G5-F5 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | JUNB RNASEHI RNHIA RNHLribonuclease H2 large subunit |
|---|---|
| Molecular Weight(MW) | 33395 Da |
| Tissue Specificity | RNASEH2A (AAH11748, 1 a.a. ~ 300 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Entrez Gene | 10535 |
|---|
Application
Western Blot analysis of RNASEH2A expression in transfected 293T cell line by RNASEH2A monoclonal antibody (M01), clone 3G5-F5.Lane 1: RNASEH2A transfected lysate(33.4 KDa).Lane 2: Non-transfected lysate.
Application
Western blot analysis of RNASEH2A over-expressed 293 cell line, cotransfected with RNASEH2A Validated Chimera RNAi ( Cat # H00010535-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNASEH2A monoclonal antibody (M01), clone 3G5-F5 (Cat # AT3653a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
