Application
Figure 1: Western blot analysis using anti-RON mAb against HCC827 (1), HT-29 (2), HCT-116 (3) and BxPC-3 (4) cell lysate.| Product Name | RON Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTK8; CD136; CDw136; MST1R |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1B5A9 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PTK8 RON |
|---|---|
| Alternative Names | PTK8 CD136 CDw136 MST1R |
| Molecular Weight(MW) | 152271 Da |
| Function | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1 Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand |
| Tissue Specificity | Expressed in colon, skin, lung and bone marrow |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 4486 |
|---|---|
| Nucleotide Accession | NP_002438.2 |
Application
Figure 1: Western blot analysis using anti-RON mAb against HCC827 (1), HT-29 (2), HCT-116 (3) and BxPC-3 (4) cell lysate.| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human RON (aa210-320) expressed in E. Coli. |
