Application
Western blot analysis of anti-ROS Pab (Cat. #ap7676a) in HL-60 cell lysate. ROS (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Product Name | ROS Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ROS1; MCF3; ROS; Proto-oncogene tyrosine-protein kinase ROS; Proto-oncogene c-Ros; Proto-oncogene c-Ros-1; Receptor tyrosine kinase c-ros oncogene 1; c-Ros receptor tyrosine kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MCF3 ROS |
|---|---|
| Alternative Names | ROS1 MCF3 ROS Proto-oncogene tyrosine-protein kinase ROS Proto-oncogene c-Ros Proto-oncogene c-Ros-1 Receptor tyrosine kinase c-ros oncogene 1 c-Ros receptor tyrosine kinase |
| Molecular Weight(MW) | 263915 Da |
| Function | Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2 |
| Tissue Specificity | Expressed in brain. Expression is increased in primary gliomas |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (Probable) |
| Entrez Gene | 6098 |
|---|
Application
Western blot analysis of anti-ROS Pab (Cat. #ap7676a) in HL-60 cell lysate. ROS (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
