Application
Figure 1: Western blot analysis using anti-S100B mAb against full-length S100B recombinant protein.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human brain (A) and human thymus tissues (B), showing cytoplasmic localization using anti-S100B mAb with DAB staining.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human brain, cerebellun using anti-S100B mAb with DAB staining.| Product Name | S100B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEF; S100; S100beta |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 9A11B9 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NEF S100 S100beta |
|---|---|
| Molecular Weight(MW) | 10713 Da |
| Function | Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization |
| Tissue Specificity | Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 6285 |
|---|---|
| Nucleotide Accession | NP_006263.1 |
Application
Figure 1: Western blot analysis using anti-S100B mAb against full-length S100B recombinant protein.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human brain (A) and human thymus tissues (B), showing cytoplasmic localization using anti-S100B mAb with DAB staining.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human brain, cerebellun using anti-S100B mAb with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of S100B expressed in E. Coli. |
