Application
The anti-UBA2 Antibody (C-term A600) is used in Western blot to detect UBA2 in human placenta tissue lysate.Application
Western blot analysis of anti-UBA2 Antibody (C-term A600) in K562 cell line lysates (35ug/lane). UBA2(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | SAE2 (UBA2) Antibody (C-term A600) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBA2; SAE2; UBLE1B; SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SAE2 UBLE1B |
|---|---|
| Alternative Names | UBA2 SAE2 UBLE1B SUMO-activating enzyme subunit 2 Anthracycline-associated resistance ARX Ubiquitin-like 1-activating enzyme E1B |
| Molecular Weight(MW) | 71224 Da |
| Function | The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 |
| Tissue Specificity | This SAE2 (UBA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 593~623 amino acids from the C-terminal region of human UBA2. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 10054 |
|---|
Application
The anti-UBA2 Antibody (C-term A600) is used in Western blot to detect UBA2 in human placenta tissue lysate.
Application
Western blot analysis of anti-UBA2 Antibody (C-term A600) in K562 cell line lysates (35ug/lane). UBA2(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
