Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SCGN antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-SCGN Antibody (C-term) in CEM cell line lysates (35ug/lane). SCGN (arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of *SCGN (arrow) using rabbit polyclonal *SCGN Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the *SCGN gene.| Product Name | SCGN Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCGN; SECRET; Secretagogin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SECRET |
|---|---|
| Alternative Names | SCGN SECRET Secretagogin |
| Molecular Weight(MW) | 32040 Da |
| Tissue Specificity | Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons |
| Cellular Localization | Cytoplasm. Secreted (By similarity). Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) Note=Predominantly cytoplasmic. A small proportion is associated with secretory granules and membrane fractions (By similarity) Detectable in human serum after ischemic neuronal damage |
| Entrez Gene | 10590 |
|---|
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SCGN antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-SCGN Antibody (C-term) in CEM cell line lysates (35ug/lane). SCGN (arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of *SCGN (arrow) using rabbit polyclonal *SCGN Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the *SCGN gene.| Application Notes | IHC:1:50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
