Application
Western blot analysis of *SCGN (arrow) using rabbit polyclonal *SCGN Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the *SCGN gene.Application
SCGN Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCGN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SCGN) Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SCGN Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCGN; SECRET; Secretagogin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SECRET |
|---|---|
| Alternative Names | SCGN SECRET Secretagogin |
| Molecular Weight(MW) | 32040 Da |
| Tissue Specificity | Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons |
| Cellular Localization | Cytoplasm. Secreted (By similarity). Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) Note=Predominantly cytoplasmic. A small proportion is associated with secretory granules and membrane fractions (By similarity) Detectable in human serum after ischemic neuronal damage |
| Entrez Gene | 10590 |
|---|
Application
Western blot analysis of *SCGN (arrow) using rabbit polyclonal *SCGN Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the *SCGN gene.
Application
SCGN Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCGN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SCGN) Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
