Application
Western blot analysis of SEMA6A Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate. SEMA6A (arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of anti-SEMA6A Antibody (C-term) pre-incubated with(right lane) and without(left lane) blocking peptide in mouse cerebellum tissue lysate. SEMA6A (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SEMA6A antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | SEMA6A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SEMA6A; KIAA1368; SEMAQ; Semaphorin-6A; Semaphorin VIA; Semaphorin-6A-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1368 SEMAQ |
|---|---|
| Alternative Names | SEMA6A KIAA1368 SEMAQ Semaphorin-6A Semaphorin VIA Semaphorin-6A-1 |
| Molecular Weight(MW) | 114395 Da |
| Function | Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation (By similarity) |
| Tissue Specificity | This SEMA6A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1001~1030 amino acids from the C-terminal region of human SEMA6A. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 57556 |
|---|
Application
Western blot analysis of SEMA6A Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate. SEMA6A (arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of anti-SEMA6A Antibody (C-term) pre-incubated with(right lane) and without(left lane) blocking peptide in mouse cerebellum tissue lysate. SEMA6A (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SEMA6A antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
