Application
The anti-SET07 Mab (Cat. #AM1191a) is used in Western blot to detect SET07 in SAOS-2 (lane 1) and NIH/3T3 cell lysates (lane 2).Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with SET07 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
(TOP)Negative control of hela cells without PE-conjugated goat anti-mouse lgG (whole molecule).PE-conjugated goat anti-mouse lgG emits red fluorescence. ( Bottom)Immunofluorescence analysis of SET07 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule).| Product Name | SET07 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SETD8; KMT5A; PRSET7; SET07; SET8; N-lysine methyltransferase SETD8; H4-K20-HMTase SETD8; Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07; SET domain-containing protein 8 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 43AT551.86 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KMT5A PRSET7 SET07 SET8 |
|---|---|
| Alternative Names | SETD8 KMT5A PRSET7 SET07 SET8 N-lysine methyltransferase SETD8 H4-K20-HMTase SETD8 Histone-lysine N-methyltransferase SETD8 Lysine N-methyltransferase 5A PR/SET domain-containing protein 07 SET domain-containing protein 8 |
| Molecular Weight(MW) | 42890 Da |
| Function | Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes |
| Tissue Specificity | This SET07 antibody was raised using purified recombinant GST fusion protein encoding the N-terminal region of human SET07. |
| Cellular Localization | Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin |
| Entrez Gene | 387893 |
|---|
Application
The anti-SET07 Mab (Cat. #AM1191a) is used in Western blot to detect SET07 in SAOS-2 (lane 1) and NIH/3T3 cell lysates (lane 2).
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with SET07 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
(TOP)Negative control of hela cells without PE-conjugated goat anti-mouse lgG (whole molecule).PE-conjugated goat anti-mouse lgG emits red fluorescence. ( Bottom)Immunofluorescence analysis of SET07 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
