Application
Western blot analysis of SFRS1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). SFRS1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SFRS1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of SFRS1 (arrow) using rabbit polyclonal SFRS1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SFRS1 gene.Application
SFRS1 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SFRS1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SRSF1; ASF; SF2; SF2P33; SFRS1; Serine/arginine-rich splicing factor 1; Alternative-splicing factor 1; Splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ASF SF2 SF2P33 SFRS1 |
|---|---|
| Alternative Names | SRSF1 ASF SF2 SF2P33 SFRS1 Serine/arginine-rich splicing factor 1 Alternative-splicing factor 1 Splicing factor arginine/serine-rich 1 pre-mRNA-splicing factor SF2 P33 subunit |
| Molecular Weight(MW) | 27745 Da |
| Function | Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'- CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors |
| Tissue Specificity | This SFRS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165~194 amino acids from the C-terminal region of human SFRS1. |
| Cellular Localization | Cytoplasm. Nucleus speckle. Note=In nuclear speckles. Shuttles between the nucleus and the cytoplasm |
| Entrez Gene | 6426 |
|---|
Application
Western blot analysis of SFRS1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). SFRS1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SFRS1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of SFRS1 (arrow) using rabbit polyclonal SFRS1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SFRS1 gene.
Application
SFRS1 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
