Application
Western blot analysis of SFRS1 Antibody (N-term) in A2058, WiDr cell line lysates (35ug/lane). SFRS1 (arrow) was detected using the purified Pab.Application
SFRS1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SFRS1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of SFRS1 (arrow) using rabbit polyclonal SFRS1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SFRS1 gene.| Product Name | SFRS1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SRSF1; ASF; SF2; SF2P33; SFRS1; Serine/arginine-rich splicing factor 1; Alternative-splicing factor 1; Splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ASF SF2 SF2P33 SFRS1 |
|---|---|
| Alternative Names | SRSF1 ASF SF2 SF2P33 SFRS1 Serine/arginine-rich splicing factor 1 Alternative-splicing factor 1 Splicing factor arginine/serine-rich 1 pre-mRNA-splicing factor SF2 P33 subunit |
| Molecular Weight(MW) | 27745 Da |
| Function | Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'- CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors |
| Tissue Specificity | This SFRS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17~47 amino acids from the N-terminal region of human SFRS1. |
| Cellular Localization | Cytoplasm. Nucleus speckle. Note=In nuclear speckles. Shuttles between the nucleus and the cytoplasm |
| Entrez Gene | 6426 |
|---|
Application
Western blot analysis of SFRS1 Antibody (N-term) in A2058, WiDr cell line lysates (35ug/lane). SFRS1 (arrow) was detected using the purified Pab.
Application
SFRS1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SFRS1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of SFRS1 (arrow) using rabbit polyclonal SFRS1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SFRS1 gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
