Application
Figure 1: Western blot analysis using anti-SKP2 monoclonal antibody against truncated Trx-SKP2 recombinant protein(1) and GST-SKP2(aa1-130) recombinant protein(2).| Product Name | SKP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6G9D10 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FBXL1 |
|---|---|
| Alternative Names | FBL1 FLB1 FBXL1 MGC1366 SKP2 |
| Molecular Weight(MW) | 47761 Da |
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1 Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration |
| Tissue Specificity | Purified recombinant fragment of SKP2(aa1-130) expressed in E. Coli. |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 6502 |
|---|---|
| Nucleotide Accession | NP_116026.1 |
Application
Figure 1: Western blot analysis using anti-SKP2 monoclonal antibody against truncated Trx-SKP2 recombinant protein(1) and GST-SKP2(aa1-130) recombinant protein(2).| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of SKP2(aa1-130) expressed in E. Coli. |
