Application
Western blot analysis of anti-SMC2 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). SMC2(arrow) was detected using the purified Pab (1:60 dilution).| Product Name | SMC2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMC2; CAPE; SMC2L1; Structural maintenance of chromosomes protein 2; Chromosome-associated protein E; XCAP-E homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAPE SMC2L1 |
|---|---|
| Alternative Names | SMC2 CAPE SMC2L1 Structural maintenance of chromosomes protein 2 Chromosome-associated protein E XCAP-E homolog |
| Molecular Weight(MW) | 135656 Da |
| Function | Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases |
| Tissue Specificity | This SMC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1168~1197 amino acids from the C-terminal region of human SMC2. |
| Cellular Localization | Nucleus. Cytoplasm. Chromosome. Note=In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation Dissociation from chromosomes is observed in late telophase |
Application
Western blot analysis of anti-SMC2 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). SMC2(arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Data sheet for OM221510