Application
SMG1 Antibody (C-term C3637)(Cat#AP8055e)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMG1 Antibody (C-term C3637) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of SMG1 Antibody (C-term C3637)(Cat#AP8055e) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Product Name | SMG1 Antibody (C-term C3637) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMG1; ATX; KIAA0421; LIP; Serine/threonine-protein kinase SMG1; 61E3.4; Lambda/iota protein kinase C-interacting protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCIF |
| IHC:1:10~50 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATX KIAA0421 LIP |
|---|---|
| Alternative Names | SMG1 ATX KIAA0421 LIP Serine/threonine-protein kinase SMG1 61E3.4 Lambda/iota protein kinase C-interacting protein |
| Molecular Weight(MW) | 410501 Da |
| Function | Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ |
| Tissue Specificity | Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver |
| Cellular Localization | Nucleus. Cytoplasm. |
| Entrez Gene | 23049 |
|---|
Application
SMG1 Antibody (C-term C3637)(Cat#AP8055e)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMG1 Antibody (C-term C3637) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of SMG1 Antibody (C-term C3637)(Cat#AP8055e) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Application Notes | IHC:1:10~50 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
