WB
WB analysis of human placenta using ENPP2 Polyclonal antibody. human placenta tissue were subjected to SDS PAGE followed by western blot with Autotaxin antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC
IHC staining of human liver cancer using ENPP2 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Autotaxin antibody at dilution of 1:50 (under 10x lens).IP
IP experiment of mouse brain using ENPP2 Polyclonal antibody. IP result of anti-Autotaxin (5ug; 1:500) with mouse brain tissue lysate 4000ug.| Product Name | ENPP2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ENPP2 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:500-1:3000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ENPP2 |
|---|---|
| Gene Synonyms | ATX NPP2 ATX-X PDNP2 LysoPLD AUTOTAXIN PD-IALPHA |
| Gene Full Name | ectonucleotide pyrophosphatase/phosphodiesterase 2 |
| Gene Summary | The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008] |
| Alternative Names | AUTOTAXIN ATX ATX X E NPP 2 EC:3.1.4.39 |
| Molecular Weight(MW) | 99 kDa(Observed band size:100-110 kDa) |
| Cellular Localization | Secreted. |
WB
WB analysis of human placenta using ENPP2 Polyclonal antibody. human placenta tissue were subjected to SDS PAGE followed by western blot with Autotaxin antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human liver cancer using ENPP2 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Autotaxin antibody at dilution of 1:50 (under 10x lens).
IP
IP experiment of mouse brain using ENPP2 Polyclonal antibody. IP result of anti-Autotaxin (5ug; 1:500) with mouse brain tissue lysate 4000ug.| Application Notes | WB:500-1:3000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
