Application
Western blot analysis of anti-ENPP2 Antibody (Center K416) in Y79 cell line lysates (35ug/lane).ENPP2(arrow) was detected using the purified Pab.Application
Western blot analysis of anti-ENPP2 Antibody (Center K416) in mouse cerebellum tissue lysates (35ug/lane).ENPP2(arrow) was detected using the purified Pab.Application
ENPP2 Antibody (Center K416) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENPP2 Antibody (Center K416) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ENPP2 Antibody (Center K416) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ENPP2 Antibody (Center K416) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ENPP2; ATX; PDNP2; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; Autotaxin; Extracellular lysophospholipase D |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATX PDNP2 |
|---|---|
| Alternative Names | ENPP2 ATX PDNP2 Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 Autotaxin Extracellular lysophospholipase D |
| Molecular Weight(MW) | 98994 Da |
| Function | Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor |
| Tissue Specificity | Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues |
| Cellular Localization | Secreted. Note=Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells |
| Entrez Gene | 5168 |
|---|
Application
Western blot analysis of anti-ENPP2 Antibody (Center K416) in Y79 cell line lysates (35ug/lane).ENPP2(arrow) was detected using the purified Pab.
Application
Western blot analysis of anti-ENPP2 Antibody (Center K416) in mouse cerebellum tissue lysates (35ug/lane).ENPP2(arrow) was detected using the purified Pab.
Application
ENPP2 Antibody (Center K416) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENPP2 Antibody (Center K416) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ENPP2 Antibody (Center K416) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
