Application
Western blot analysis of SMYD3 antibody (Center) in CEM cell line lysates (35ug/lane). SMYD3 (arrow) was detected using the purified Pab.Application
SMYD3 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SMYD3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMYD3; ZMYND1; ZNFN3A1; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ZMYND1 ZNFN3A1 |
|---|---|
| Alternative Names | SMYD3 ZMYND1 ZNFN3A1 SET and MYND domain-containing protein 3 Zinc finger MYND domain-containing protein 1 |
| Molecular Weight(MW) | 49097 Da |
| Function | Histone methyltransferase. Specifically methylates 'Lys- 4' and 'Lys-5' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences |
| Tissue Specificity | Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas |
| Cellular Localization | Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M |
| Entrez Gene | 64754 |
|---|
Application
Western blot analysis of SMYD3 antibody (Center) in CEM cell line lysates (35ug/lane). SMYD3 (arrow) was detected using the purified Pab.
Application
SMYD3 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
