Application
Western blot analysis of SMYD3 antibody (N-term) in CEM cell line lysates (35ug/lane). SMYD3 (arrow) was detected using the purified Pab.Application
SMYD3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMYD3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SMYD3 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SMYD3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMYD3; ZMYND1; ZNFN3A1; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ZMYND1 ZNFN3A1 |
|---|---|
| Alternative Names | SMYD3 ZMYND1 ZNFN3A1 SET and MYND domain-containing protein 3 Zinc finger MYND domain-containing protein 1 |
| Molecular Weight(MW) | 49097 Da |
| Function | Histone methyltransferase. Specifically methylates 'Lys- 4' and 'Lys-5' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences |
| Tissue Specificity | Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas |
| Cellular Localization | Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M |
| Entrez Gene | 64754 |
|---|
Application
Western blot analysis of SMYD3 antibody (N-term) in CEM cell line lysates (35ug/lane). SMYD3 (arrow) was detected using the purified Pab.
Application
SMYD3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMYD3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SMYD3 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
