Application
Figure 1: Western blot analysis using anti-SOD1 mAb against Hela (1), NIH/3T3 (2), A549 (3) and A431 (4) cell lysate.Application
Figure 2: Confocal immunofluorescence analysis of PANC-1 (left) and SKBR-3 (right) cells using anti-SOD1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Application
Figure 3: Confocal immunofluorescence analysis of 3T3-L1 cells using anti-SOD1 mAb (green). Blue: DRAQ5 fluorescent DNA dye.| Product Name | SOD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALS; SOD; ALS1; IPOA; homodimer |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6F5 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ALS SOD ALS1 IPOA homodimer |
|---|---|
| Molecular Weight(MW) | 15936 Da |
| Function | Destroys radicals which are normally produced within the cells and which are toxic to biological systems |
| Tissue Specificity | Purified recombinant fragment of human SOD1 expressed in E. Coli. |
| Cellular Localization | Cytoplasm. Note=The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria |
| Entrez Gene | 6647 |
|---|---|
| Nucleotide Accession | NP_000445.1 |
Application
Figure 1: Western blot analysis using anti-SOD1 mAb against Hela (1), NIH/3T3 (2), A549 (3) and A431 (4) cell lysate.
Application
Figure 2: Confocal immunofluorescence analysis of PANC-1 (left) and SKBR-3 (right) cells using anti-SOD1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 3: Confocal immunofluorescence analysis of 3T3-L1 cells using anti-SOD1 mAb (green). Blue: DRAQ5 fluorescent DNA dye.| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human SOD1 expressed in E. Coli. |
