Application
SOCS4 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the SOCS4 antibody detected the SOCS4 protein (arrow).Application
SOCS4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SOCS4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of SOCS4 Antibody (Center)(Cat#AP11847c) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | SOCS4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SOCS4; SOCS7; Suppressor of cytokine signaling 4; Suppressor of cytokine signaling 7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SOCS7 |
|---|---|
| Alternative Names | SOCS4 SOCS7 Suppressor of cytokine signaling 4 Suppressor of cytokine signaling 7 |
| Molecular Weight(MW) | 50623 Da |
| Function | SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction Substrate-recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits EGF signaling by mediating the degradation of the Tyr-phosphorylated EGF receptor/EGFR |
| Tissue Specificity | This SOCS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-262 amino acids from the Central region of human SOCS4. |
| Entrez Gene | 122809 |
|---|---|
| Nucleotide Accession | NP_955453.1 NP_543143.1 |
Application
SOCS4 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the SOCS4 antibody detected the SOCS4 protein (arrow).
Application
SOCS4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SOCS4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of SOCS4 Antibody (Center)(Cat#AP11847c) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
