Application
Fluorescent confocal image of an SY5Y cell stained with AM2048b SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AM2048b SOX2 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). SOX2 immunoreactivity is localized exclusively to the nucleus.Application
Western blot analysis of anti-SOX2 Monoclonal Antibody(Cat.#AM2048b) by human SOX2 recombinant protein cell line lysate (35ug/lane). SOX2 (His tagged) (arrow) was detected using the purified Mab.Application
Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (ascites)(bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.| Product Name | SOX2 Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SOX2; Transcription factor SOX-2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 57CT23.3.4 |
| Host Species | Mouse |
| Tested Applications | IFWBFC |
| IF:1:200 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SOX2 Transcription factor SOX-2 |
|---|---|
| Molecular Weight(MW) | 34310 Da |
| Function | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity) |
| Tissue Specificity | Human SOX2 recombinant protein is used to produce this monoclonal antibody. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6657 |
|---|
Application
Fluorescent confocal image of an SY5Y cell stained with AM2048b SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AM2048b SOX2 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). SOX2 immunoreactivity is localized exclusively to the nucleus.
Application
Western blot analysis of anti-SOX2 Monoclonal Antibody(Cat.#AM2048b) by human SOX2 recombinant protein cell line lysate (35ug/lane). SOX2 (His tagged) (arrow) was detected using the purified Mab.
Application
Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (ascites)(bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.| Application Notes | IF:1:200 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
