Application
Western blot analysis of SPARC Antibody (C-term) in Y79 cell line lysates (35ug/lane). SPARC (arrow) was detected using the purified Pab.Application
Western blot analysis of SPARC (arrow) using rabbit polyclonal SPARC Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of widr cells using SPARC Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SPARC Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPARC; ON; Basement-membrane protein 40; Osteonectin; Secreted protein acidic and rich in cysteine |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ON |
|---|---|
| Alternative Names | SPARC ON Basement-membrane protein 40 Osteonectin Secreted protein acidic and rich in cysteine |
| Molecular Weight(MW) | 34632 Da |
| Function | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity |
| Tissue Specificity | This SPARC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230~260 amino acids from the C-terminal region of human SPARC. |
| Cellular Localization | Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane |
| Entrez Gene | 6678 |
|---|
Application
Western blot analysis of SPARC Antibody (C-term) in Y79 cell line lysates (35ug/lane). SPARC (arrow) was detected using the purified Pab.
Application
Western blot analysis of SPARC (arrow) using rabbit polyclonal SPARC Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.
Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of widr cells using SPARC Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
