Application
SPARC Antibody (C-term)(Ascites)(Cat. #AM2175a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPARC antibody detected the SPARC protein (arrow).| Product Name | SPARC (C-term)(Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPARC; ON; Basement-membrane protein 40; Osteonectin; Secreted protein acidic and rich in cysteine |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 664CT7.3.1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100~800 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ON |
|---|---|
| Alternative Names | SPARC ON Basement-membrane protein 40 Osteonectin Secreted protein acidic and rich in cysteine |
| Molecular Weight(MW) | 34632 Da |
| Function | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity |
| Tissue Specificity | This SPARC antibody is generated from mouses immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC. |
| Cellular Localization | Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane |
Application
SPARC Antibody (C-term)(Ascites)(Cat. #AM2175a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPARC antibody detected the SPARC protein (arrow).| Application Notes | WB:1:100~800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
