Application
Western blot analysis of SPG20 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). SPG20 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SPG20 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
SPG20 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SPG20 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPG20; KIAA0610; TAHCCP1; Spartin; Spastic paraplegia 20 protein; Trans-activated by hepatitis C virus core protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0610 TAHCCP1 |
|---|---|
| Alternative Names | SPG20 KIAA0610 TAHCCP1 Spartin Spastic paraplegia 20 protein Trans-activated by hepatitis C virus core protein 1 |
| Molecular Weight(MW) | 72833 Da |
| Function | May be implicated in endosomal trafficking, or microtubule dynamics, or both |
| Tissue Specificity | Ubiquitously expressed, with highest levels of expression detected in adipose tissue |
| Cellular Localization | Cytoplasm. Note=Transiently associated with endosomes |
| Entrez Gene | 23111 |
|---|
Application
Western blot analysis of SPG20 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). SPG20 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SPG20 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
SPG20 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
