Application
Western blot analysis of SPHK1 Antibody (C-term R414) in 293 cell line lysates (35ug/lane).SPHK1 (arrow) was detected using the purified Pab.Application
SPHK1 Antibody (C-term R414) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPHK1 Antibody (C-term R414) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (C-term R414) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.Application
SPHK1 Antibody (C-term R414) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SPHK1 Antibody (C-term R414) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPHK1; SPHK; SPK; Sphingosine kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SPHK SPK |
|---|---|
| Alternative Names | SPHK1 SPHK SPK Sphingosine kinase 1 |
| Molecular Weight(MW) | 42518 Da |
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol |
| Tissue Specificity | Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Western blot analysis of SPHK1 Antibody (C-term R414) in 293 cell line lysates (35ug/lane).SPHK1 (arrow) was detected using the purified Pab.
Application
SPHK1 Antibody (C-term R414) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPHK1 Antibody (C-term R414) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (C-term R414) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.
Application
SPHK1 Antibody (C-term R414) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
