Application
The anti-SphK1 Pab is used in Western blot (Lane 2) to detect c-myc-tagged SphK1 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 1). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH).Application
Western blot analysis of anti-hSPHK1-M1 Pab in 293 cell line lysates transiently transfected with the SPHK1 gene (2ug/lane). hSPHK1-M1(arrow) was detected using the purified Pab (1:60 dilution).| Product Name | SPHK1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPHK1; SPHK; SPK; Sphingosine kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SPHK SPK |
|---|---|
| Alternative Names | SPHK1 SPHK SPK Sphingosine kinase 1 |
| Molecular Weight(MW) | 42518 Da |
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol |
| Tissue Specificity | Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 8877 |
|---|
Application
The anti-SphK1 Pab is used in Western blot (Lane 2) to detect c-myc-tagged SphK1 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 1). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH).
Application
Western blot analysis of anti-hSPHK1-M1 Pab in 293 cell line lysates transiently transfected with the SPHK1 gene (2ug/lane). hSPHK1-M1(arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
