Application
The anti-SPHK1 Pab is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.Application
Western blot analysis of anti-SPHK1 Antibody (Center) in 293 cell line lysates (35ug/lane). SPHK1 (arrow) was detected using the purified Pab.Application
Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (R301) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.| Product Name | SPHK1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPHK1; SPHK; SPK; Sphingosine kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SPHK SPK |
|---|---|
| Alternative Names | SPHK1 SPHK SPK Sphingosine kinase 1 |
| Molecular Weight(MW) | 42518 Da |
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol |
| Tissue Specificity | Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 8877 |
|---|
Application
The anti-SPHK1 Pab is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.
Application
Western blot analysis of anti-SPHK1 Antibody (Center) in 293 cell line lysates (35ug/lane). SPHK1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (R301) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
