Application
Western blot analysis of SYTL2 Antibody (Center) in Hela cell line lysates (35ug/lane). SYTL2 (arrow) was detected using the purified Pab.Application
SYTL2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYTL2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SYTL2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SYTL2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SYTL2; KIAA1597; SGA72M; SLP2; SLP2A; Synaptotagmin-like protein 2; Breast cancer-associated antigen SGA-72M; Exophilin-4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1597 SGA72M SLP2 SLP2A |
|---|---|
| Alternative Names | SYTL2 KIAA1597 SGA72M SLP2 SLP2A Synaptotagmin-like protein 2 Breast cancer-associated antigen SGA-72M Exophilin-4 |
| Molecular Weight(MW) | 104930 Da |
| Function | Isoform 1 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. It is required for cytotoxic granule docking at the immunologic synapse Isoform 4 binds phosphatidylserine (PS) and phosphatidylinositol- 4,5-bisphosphate (PIP2) and promotes the recruitment of glucagon- containing granules to the cell membrane in pancreatic alpha cells. Binding to PS is inhibited by Ca(2+) while binding to PIP2 is Ca(2+) insensitive |
| Tissue Specificity | Isoform 1 is expressed in hematopoietic lineages with a strong expression in CD4 and CD8 T-lymphocytes. It is also widely expressed in nonhematopoietic tissues. Isoform 5 is expressed only in nonhematopoietic tissues. Isoform 4 is expressed in pancreatic alpha cells |
| Cellular Localization | Isoform 1: Cytoplasm. Cell membrane. Note=Recruited on vesicular structures in cytotoxic T-lymphocytes (CTL) by RAB27A |
| Entrez Gene | 54843 |
|---|
Application
Western blot analysis of SYTL2 Antibody (Center) in Hela cell line lysates (35ug/lane). SYTL2 (arrow) was detected using the purified Pab.
Application
SYTL2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYTL2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SYTL2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
