Application
Western blot analysis of T over-expressed 293 cell line, cotransfected with T Validated Chimera RNAi ( Cat # H00006862-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with T monoclonal antibody (M01), clone 5H8 (Cat # AT4130a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Application
Western Blot analysis of T expression in transfected 293T cell line by T monoclonal antibody (M01), clone 5H8.Lane 1: T transfected lysate(41.1 KDa).Lane 2: Non-transfected lysate.| Product Name | T Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC104817, TFTT, brachyury homolog (mouse) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 5H8 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MGC104817 TFTT brachyury homolog (mouse) |
|---|---|
| Molecular Weight(MW) | 47443 Da |
| Tissue Specificity | T (NP_003172, 222 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Application
Western blot analysis of T over-expressed 293 cell line, cotransfected with T Validated Chimera RNAi ( Cat # H00006862-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with T monoclonal antibody (M01), clone 5H8 (Cat # AT4130a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Application
Western Blot analysis of T expression in transfected 293T cell line by T monoclonal antibody (M01), clone 5H8.Lane 1: T transfected lysate(41.1 KDa).Lane 2: Non-transfected lysate.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
