Application
Western blot analysis on SH-SY5Y cell lysates using anti-Phospho-Tau (pS404) RabMAb (Cat. #AJ1757e). Cells were either (A) untreated (B) treated with Okadaic Acid and Calyculin A.Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Phospho-specific anti-Tau RabMAb (Cat. #AJ1757e).| Product Name | Tau Antibody Phospho (pS404) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAPT, MAPTL, MTBT1, TAU, Microtubule-associated protein tau, Neurofibrillary tangle protein;Paired helical filament-tau |
| Modification | p-S404 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2605 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAPTL MTBT1 TAU |
|---|---|
| Alternative Names | MAPT MAPTL MTBT1 TAU Microtubule-associated protein tau Neurofibrillary tangle protein Paired helical filament-tau |
| Molecular Weight(MW) | 78928 Da |
| Function | Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization |
| Tissue Specificity | Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system |
| Cellular Localization | Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Note=Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components |
| Entrez Gene | 4137 |
|---|
Application
Western blot analysis on SH-SY5Y cell lysates using anti-Phospho-Tau (pS404) RabMAb (Cat. #AJ1757e). Cells were either (A) untreated (B) treated with Okadaic Acid and Calyculin A.
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Phospho-specific anti-Tau RabMAb (Cat. #AJ1757e).| Application Notes | WB:1:1000~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
