Application
Western blot analysis of TERA Antibody (Cat. #AM1841a) in Jurkat cell line lysates (35ug/lane).TERA (arrow) was detected using the purified Mab.(1:1000)Application
TERA Monoclonal Antibody (Ascites) (Cat. #AM1841a ) immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TERA Monoclonal Antibody (Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.Application
TERA Monoclonal Antibody (Ascites) (Cat. #AM1841a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.| Product Name | TERA Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VCP; Transitional endoplasmic reticulum ATPase; 15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1G9E2B6 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | VCP Transitional endoplasmic reticulum ATPase 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein |
|---|---|
| Molecular Weight(MW) | 89322 Da |
| Function | Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope Regulates E3 ubiquitin-protein ligase activity of RNF19A (By similarity). Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168- dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage |
| Tissue Specificity | This TERA antibody is generated from mouse immunized with TERA recombinant protein. |
| Cellular Localization | Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus. Note=Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients. Recruited to the cytoplasmic surface of the endoplasmic reticulum via interaction with AMFR/gp78. Following DNA double-strand breaks, recruited to the sites of damage. Recruited to stalled replication forks via interaction with SPRTN |
| Entrez Gene | 7415 |
|---|
Application
Western blot analysis of TERA Antibody (Cat. #AM1841a) in Jurkat cell line lysates (35ug/lane).TERA (arrow) was detected using the purified Mab.(1:1000)
Application
TERA Monoclonal Antibody (Ascites) (Cat. #AM1841a ) immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TERA Monoclonal Antibody (Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
TERA Monoclonal Antibody (Ascites) (Cat. #AM1841a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.| Application Notes | WB:1:500-1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
