Application
Western blot analysis of TGIF1 (arrow) using rabbit polyclonal TGIF1 Antibody (Center A160) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene.| Product Name | TGIF1 Antibody (Center A160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TGIF |
|---|---|
| Alternative Names | TGIF1 TGIF Homeobox protein TGIF1 5'-TG-3'-interacting factor 1 |
| Molecular Weight(MW) | 43013 Da |
| Function | Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII- RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities |
| Tissue Specificity | This TGIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-182 amino acids from the Central region of human TGIF1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 7050 |
|---|---|
| Nucleotide Accession | NP_733796.2 NP_003235.1 |
Application
Western blot analysis of TGIF1 (arrow) using rabbit polyclonal TGIF1 Antibody (Center A160) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene.| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
