Application
THOC1 Antibody (N-term) (Cat. #AM1950b) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the THOC1 antibody detected the THOC1 protein (arrow).| Product Name | THOC1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | 301CT40.5.1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100~250 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HPR1 |
|---|---|
| Alternative Names | THOC1 HPR1 THO complex subunit 1 Nuclear matrix protein p84 hTREX84 |
| Molecular Weight(MW) | 75666 Da |
| Function | Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4 |
| Tissue Specificity | Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression |
| Cellular Localization | Isoform 1: Nucleus speckle. Nucleus, nucleoplasm. Nucleus matrix. Cytoplasm. Note=Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution |
| Entrez Gene | 9984 |
|---|---|
| Nucleotide Accession | NP_005122.2 |
Application
THOC1 Antibody (N-term) (Cat. #AM1950b) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the THOC1 antibody detected the THOC1 protein (arrow).| Application Notes | WB:1:100~250 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
