Application
p84 antibody [5E10] detects p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p84 protein stained by p84 antibody [5E10] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Application
p84 antibody [5E10] detects p84 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5% SDS-PAGE p84 antibody [5E10] dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Detection of human p84/N5 in nuclear fraction by anti-p84/N5 5E10 monoclonal antibody in western blot experiment. Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.Application
Sample (30 µg of whole cell lysate) A: HeLa B: HeLa nucleus 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse Cerebellum 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: 293T B: NIH-3T3 C: mouse brain D: PC-12 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 μg of p84 antibody [5E10] 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Product Name | Nuclear Matrix Protein p84 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [5E10] to Nuclear Matrix Protein p84P84/N5-5E10 recognizes an 84 kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies. |
| Immunogen | Amino acids 15-374 of human p84 expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 5E10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWBChIP |
| Recommended Starting Dilutions: For ICC/IF: Use at a concentration of 0.5 - 2 µg/ml. For IP: 1:100-1:500. For WB: Use at a concentration of 0.3 - 2 µg/ml. For ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseMonkeyRatHamster |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HPR1 P84 P84N5 |
|---|---|
| Target | p84 |
| Molecular Weight(MW) | 84 kDa (note) |
| Tissue Specificity | Knockdown/Knockout validation was supported by references (PMID:25296641) |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
p84 antibody [5E10] detects p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p84 protein stained by p84 antibody [5E10] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Application
p84 antibody [5E10] detects p84 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5% SDS-PAGE p84 antibody [5E10] dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Detection of human p84/N5 in nuclear fraction by anti-p84/N5 5E10 monoclonal antibody in western blot experiment. Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.
Application
Sample (30 µg of whole cell lysate) A: HeLa B: HeLa nucleus 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse Cerebellum 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: 293T B: NIH-3T3 C: mouse brain D: PC-12 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 μg of p84 antibody [5E10] 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Positive Control | 293T , A431 , HeLa , HepG2 , A375 , HeLa nucleus , NIH-3T3 , mouse brain , PC-12 , mouse Cerebellum |
|---|---|
| Application Notes | Recommended Starting Dilutions: For ICC/IF: Use at a concentration of 0.5 - 2 µg/ml. For IP: 1:100-1:500. For WB: Use at a concentration of 0.3 - 2 µg/ml. For ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
