Application
TIMP1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TIMP1 antibody detected the TIMP1 protein (arrow).Application
TIMP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TIMP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of TIMP1 Antibody (C-term)(Cat#AP11199b) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Confocal immunofluorescent analysis of TIMP1 Antibody (C-term)(Cat#AP11199b) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).Application
TIMP1 Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TIMP1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CLGI, TIMP |
|---|---|
| Alternative Names | TIMP1 CLGI TIMP Metalloproteinase inhibitor 1 Erythroid-potentiating activity Fibroblast collagenase inhibitor Tissue inhibitor of metalloproteinases 1 |
| Molecular Weight(MW) | 23171 Da |
| Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14 |
| Tissue Specificity | This TIMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-195 amino acids from the C-terminal region of human TIMP1. |
| Cellular Localization | Secreted. |
Application
TIMP1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TIMP1 antibody detected the TIMP1 protein (arrow).
Application
TIMP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TIMP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of TIMP1 Antibody (C-term)(Cat#AP11199b) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Confocal immunofluorescent analysis of TIMP1 Antibody (C-term)(Cat#AP11199b) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Application
TIMP1 Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
