Application
TLE1 Antibody (Center) (Cat. #AM1982b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TLE1 antibody detected the TLE1 protein (arrow).| Product Name | TLE1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 381CT18.5.1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100~250 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TLE1 Transducin-like enhancer protein 1 E(Sp1) homolog Enhancer of split groucho-like protein 1 |
|---|---|
| Molecular Weight(MW) | 83201 Da |
| Function | Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG |
| Tissue Specificity | In all tissues examined, mostly in brain, liver and muscle |
| Cellular Localization | Nucleus. Note=Nuclear and chromatin- associated, depending on isoforms and phosphorylation status Hyperphosphorylation decreases the affinity for nuclear components |
| Entrez Gene | 7088 |
|---|---|
| Nucleotide Accession | NP_005068.2 |
Application
TLE1 Antibody (Center) (Cat. #AM1982b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TLE1 antibody detected the TLE1 protein (arrow).| Application Notes | WB:1:100~250 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
