Application
Western Blot analysis of TLR4 expression in transfected 293T cell line by TLR4 monoclonal antibody (M03), clone 1H7.Lane 1: TLR4 transfected lysate(73.3 KDa).Lane 2: Non-transfected lysate.Application
Western blot analysis of TLR4 over-expressed 293 cell line, cotransfected with TLR4 Validated Chimera RNAi ( Cat # H00007099-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TLR4 monoclonal antibody (M03), clone 1H7 (Cat # AT4256a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Product Name | TLR4 Antibody (monoclonal) (M03) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 1H7 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TOLL hTolltoll-like receptor 4 |
|---|---|
| Molecular Weight(MW) | 95680 Da |
| Tissue Specificity | TLR4 (NP_612564, 214 a.a. ~ 292 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Application
Western Blot analysis of TLR4 expression in transfected 293T cell line by TLR4 monoclonal antibody (M03), clone 1H7.Lane 1: TLR4 transfected lysate(73.3 KDa).Lane 2: Non-transfected lysate.
Application
Western blot analysis of TLR4 over-expressed 293 cell line, cotransfected with TLR4 Validated Chimera RNAi ( Cat # H00007099-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TLR4 monoclonal antibody (M03), clone 1H7 (Cat # AT4256a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
