Application
TRAF2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TRAF2 antibody detected the TRAF2 protein (arrow).Application
Western blot analysis of TRAF2 (arrow) using rabbit polyclonal TRAF2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TRAF2 gene.Application
TRAF2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRAF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | TRAF2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRAF2; TRAP3; TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TRAP3 |
|---|---|
| Alternative Names | TRAF2 TRAP3 TNF receptor-associated factor 2 E3 ubiquitin-protein ligase TRAF2 Tumor necrosis factor type 2 receptor-associated protein 3 |
| Molecular Weight(MW) | 55859 Da |
| Function | Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis Required for normal antibody isotype switching from IgM to IgG Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'- linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin- protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain |
| Tissue Specificity | This TRAF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-207 amino acids from the Central region of human TRAF2. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 7186 |
|---|---|
| Nucleotide Accession | NP_066961.2 |
Application
TRAF2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TRAF2 antibody detected the TRAF2 protein (arrow).
Application
Western blot analysis of TRAF2 (arrow) using rabbit polyclonal TRAF2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TRAF2 gene.
Application
TRAF2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRAF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
